I n vivo antiinflammatory activity and chemical composition of H ypericum scabroides

Abstract

To evaluate the methanolic extracts of aerial parts of Hypericum scabroides (HSM) (200mg/kg, p.o.) for in vivo anti-inflammatory activity. Methods: The anti-inflammatory activity of HSM was tested in mice weighting (25+-5)g. Either vehicle (control group), the methanolic extracts (200 mg/kg) or diclofenac (50 mg/kg), was administered (p.o.) for 60 min before an edema was induced in the mice paw by subcutaneous injection of carrageenin. The mouse-paw volume was measured 1 h, 3h and 6 h after injection of carrageenin. Results: The HSM showed significant reduction of edema in carrageenan induced mice paw edema model at 1h and 3h for (78.03+-15.54)%

*Corresponding author : Sadia Mansour , Phar macognos y, Api -Phyt ot er aphy Laboratory, Department of Biology, Faculty of SNV, Mostaganem University, Algeria.

E-mail: mansoursadia@gmail.com

Foundation Project: Supported by the Algerian Ministry of the Higher Education and Scientific Research, CNEPRU project approved in 2011/2013 (Grant No. F02220120001). and (40.44+-16.36)% , respectively. The diclofenac 50 mg/kg exhibited % reduction in paw volume (31.00+-11.52)% Aging, skin disorders, rheumatoid arthritis, atherosclerosis are caused by oxidative stress, occurring when production of reactive oxygen species(ROS)-in the form of hydroxyl radical, superoxide anion, peroxyl radical, singlet oxygen, hydrogen peroxi de or ozone, exceeds t he ant i oxi dant protective capacity of target cells Free radicals are the initiators of a redox reaction cascade, resulting in changes of the chemical structure of biological macromolecules, such as proteins, lipids and DNA , or disturbances of human cell metabolism [5][4], or even tissue injury Human skin is exposed to both external factors, such as radiation, smoking, pollutants, organic solvents, pesticides [6] and internal ROS products from normal cell metabolism, normal aerobic respiration, stimulated polymorphonuclear leukocytes or macrophages [8,9] , (0.80+-0.09)% and (9.39+-1.99)% after 1h, 3 h and 6 h, respectively compared to control group. The obtained results revealed that HSM has significant anti inflammatory activity. Furethermore, the chemical composition of HSM was analyzed by using high performance liquid chromatography–diode array dedector. The plant contained pseudohypericin (trace) hypericin (trace), chlorogenic acid (0.014 0+-0.000 5)% (0.005 0+-0.000 6)% , hyperoside (0.016+-0.005)% , isoquercitrin (0.034 0+-0.000 5)% and kaempferol (trace). Conclusions: The obtained results of the present investigation revealed that methanol extract of Hypericum scarbroides has significant anti-inflammatory activity