http://dspace.univ-usto.dz/handle/123456789/51 2026-04-20T16:51:17Z 2026-04-20T16:51:17Z SELAMI Nawel http://dspace.univ-usto.dz/handle/123456789/257 2015-10-18T12:34:23Z 2015-10-18T00:00:00Z

Titre: Etude des Associations Symbiotiques de Retama monosperma : Approches Morphologique, Anatomique et Ultrastructurale, Caractérisation moléculaire des Isolats Auteur(s): SELAMI Nawel Résumé: R. monosperma est une légumineuse arbustive capable d’établir une symbiose fixatrice d'azote avec des bactéries du sol, Rhizobia. Cette interaction aboutit à la formation de nodules au niveau du système racinaire de la plante. Comme l’étude de l’interaction symbiotique entre R. monosperma et les Rhizobia n’a pas été largement étudiée et Compte tenu du rôle primordial que joue cette espèce dans la bio-fertilisation et la stabilisation des dunes mobiles, nous nous sommes proposés dans la présente thèse d’établir la première étude détaillée de la morphologie et l’anatomie des nodules de R. monosperma. En effet, des nodules sauvages collectés des dunes côtières d’Oran, ont fait l’objet d’une (i) étude anatomique et ultrastructurale par microscopie photonique et électronique ; (ii) analyse génétique des microsymbiotes basée sur les séquences de gènes ARNr 16S, atpD, glnII et recA ; (iii) analyse de l’endoréduplication chez R. monosperma par cytométrie en flux. La caractérisation génétique des microsymbiotes de R. monosperma, indique que ces bactéries sont taxonomiquement très proche, voir identique aux microsymbiotes de R. sphaerocarpa récemment identifiés par Guerrouj et al. (2013), et nommée Bradyrhizobium retamae. L’étude anatomique et ultrastructurale révéle que les nodules de R. monosperma sont à croissance indéterminée, de type genistoide. Le cordon d’infection typique de la majorité des legumineuses est absent dans les nodules de R. monosperma et la diffusion de l’infection est assurée par la division des cellules nouvellement infectées. La zone fixatrice d’azote est homogène aux cellules complètement infectées et le réseau de cellules non envahi commun à la plupart des légumineuses est absent. De plus, le taux d’endoréduplication indispensable pour la différenciation des bactéroïdes dans les nodules indéterminés est trop faible voir nul dans les nodules de R. monosperma. En outre, plusieurs bactéries bénéfiques comme Methylobacterium et Pseudomonas koreensis ont été isolées à partir de nodules racinaires de R. monosperma. Dans cette étude, les premiers stades du processus de colonisation par Methylobacterium sp et P. koreensis, sont visualisés conjointement au microscope photonique et électronique. Des résultats préliminaires, témoignent clairement que P. koreensis et Methylobacterium sp se comportent comme bactéries endophytes associatives, qui peuvent nicher à l’intérieur des tissus de R. monosperma, sans lui induire de maladies symptomatiques.

2015-10-18T00:00:00Z Mansour, Sadia http://dspace.univ-usto.dz/handle/123456789/241 2015-07-07T12:25:36Z 2015-07-07T00:00:00Z

Titre: I n vivo antiinflammatory activity and chemical composition of H ypericum scabroides Auteur(s): Mansour, Sadia Résumé: To evaluate the methanolic extracts of aerial parts of Hypericum scabroides (HSM) (200mg/kg, p.o.) for in vivo anti-inflammatory activity. Methods: The anti-inflammatory activity of HSM was tested in mice weighting (25+-5)g. Either vehicle (control group), the methanolic extracts (200 mg/kg) or diclofenac (50 mg/kg), was administered (p.o.) for 60 min before an edema was induced in the mice paw by subcutaneous injection of carrageenin. The mouse-paw volume was measured 1 h, 3h and 6 h after injection of carrageenin. Results: The HSM showed significant reduction of edema in carrageenan induced mice paw edema model at 1h and 3h for (78.03+-15.54)% *Corresponding author : Sadia Mansour , Phar macognos y, Api -Phyt ot er aphy Laboratory, Department of Biology, Faculty of SNV, Mostaganem University, Algeria. E-mail: mansoursadia@gmail.com Foundation Project: Supported by the Algerian Ministry of the Higher Education and Scientific Research, CNEPRU project approved in 2011/2013 (Grant No. F02220120001). and (40.44+-16.36)% , respectively. The diclofenac 50 mg/kg exhibited % reduction in paw volume (31.00+-11.52)% Aging, skin disorders, rheumatoid arthritis, atherosclerosis are caused by oxidative stress, occurring when production of reactive oxygen species(ROS)-in the form of hydroxyl radical, superoxide anion, peroxyl radical, singlet oxygen, hydrogen peroxi de or ozone, exceeds t he ant i oxi dant protective capacity of target cells Free radicals are the initiators of a redox reaction cascade, resulting in changes of the chemical structure of biological macromolecules, such as proteins, lipids and DNA , or disturbances of human cell metabolism [5][4], or even tissue injury Human skin is exposed to both external factors, such as radiation, smoking, pollutants, organic solvents, pesticides [6] and internal ROS products from normal cell metabolism, normal aerobic respiration, stimulated polymorphonuclear leukocytes or macrophages [8,9] , (0.80+-0.09)% and (9.39+-1.99)% after 1h, 3 h and 6 h, respectively compared to control group. The obtained results revealed that HSM has significant anti inflammatory activity. Furethermore, the chemical composition of HSM was analyzed by using high performance liquid chromatography–diode array dedector. The plant contained pseudohypericin (trace) hypericin (trace), chlorogenic acid (0.014 0+-0.000 5)% (0.005 0+-0.000 6)% , hyperoside (0.016+-0.005)% , isoquercitrin (0.034 0+-0.000 5)% and kaempferol (trace). Conclusions: The obtained results of the present investigation revealed that methanol extract of Hypericum scarbroides has significant anti-inflammatory activity

2015-07-07T00:00:00Z Fodil M Benzaoui A Zemani-Fodil F Aberkane M Boughrara W Saidi-Mehtar N Petit-Teixeira E Boudjema A http://dspace.univ-usto.dz/handle/123456789/218 2015-06-09T10:01:09Z 2015-06-06T00:00:00Z

Titre: Association of PTPN22 (rs2476601) and STAT4 (rs7574865) polymorphisms with rheumatoid arthritis in the Western Algerian population Auteur(s): Fodil M; Benzaoui A; Zemani-Fodil F; Aberkane M; Boughrara W; Saidi-Mehtar N; Petit-Teixeira E; Boudjema A Résumé: Aim: The aim of the present study was to replicate the association of five risk gene polymorphisms (PTPN22- -rs2476601, STAT4-rs7574865, 6q23-rs6927172, IRF5-rs2004640 and TRAF1/C5-rs10818488) with RA in a specific population of the Western Algeria. Material and methods: The study group comprised 110 patients with RA and 197 ethnically matched heal thy control subjects. All polymorphisms were genotyped using predesigned TaqMan® assays. Allele and genotype frequencies in patients and control subjects were compared by chi-square test and odds ratios with 95% confidence intervals. Correction for multiple testing was carried out using the Bonferroni adjustment. Results: Statistically significant associations with RA were detected. The strongest signal was obtained for PTPN22-rs2476601 with an allelic Pvalue 3.32 x 10 (OR = 9.83, 95% CI [4.28 – 22.56]). A second significant association was obtained with STAT4-rs7574865 (allelic Pvalue = 4x10; OR = 1.75, 95% CI [1.16-2.63]).The third SNP, 6q23-rs6927172, showed a significant result of association with RA, but missed our criteria for significance at allelic level after Bonferroni’s correction (allelic Pvalue =0.027; OR =0.64,95% CI [0.42– 0.97]). Finally, IRF5-rs2004640 and TRAF1/C5-rs10818488 showed a significant association only at genotypic level (Pvalues: 3x10-3 and 2.9 x10-3-4 respectively) but did not reach statistical significance when comparing allele frequencies (Pvalues: 0.96 and 0.21 respectively). Conclusions: From this initial study, we can conclude that PTPN22-rs2476601 and STAT4-rs7574865 poly- -11 morphisms are clearly associated with the risk of RA in the Western Algerian population.

2015-06-06T00:00:00Z Sediki, Fatima Zohra Radoui, Abdelkarim Cabet, Faiza Zemani-Fodil, Faouzia Saidi-Mehtar, Nadhira Boudjema, Abdallah http://dspace.univ-usto.dz/handle/123456789/214 2015-06-01T09:50:21Z 2015-06-01T00:00:00Z

Titre: Detection of CFTR mutations using PCR/ARMS in a sample of Algerian population Auteur(s): Sediki, Fatima Zohra; Radoui, Abdelkarim; Cabet, Faiza; Zemani-Fodil, Faouzia; Saidi-Mehtar, Nadhira; Boudjema, Abdallah Résumé: Cystic fibrosis (CF) is the most common autosomal recessive disease in Caucasians. Wrongly considered as a European disease, CF is found in Algeria; but the literature data on the clinical profile and the spectrum of CFTR gene mutations are poor. In this study we investigate twenty-four unrelated Algerian families, with at least one child with CF. DNA extracts from blood samples of patients and parents were screened for CFTR gene mutations using Elucigene CF30 Kit which is based on a PCR/ARMS technique. Only five different mutations were identified. On the 48 alleles studied, most com- mon mutations were: c.1521_1523delCTT (F508del) 18.75%, c.579+1G>T (711+1G>T) 12.5%, c.1624G>T (G542X) 10.41%, c.3909C>G (N1303K) 4%, and c.1652G>A (G551D) 2%. The Elucigene CF30 kit highlights a portion of CFTR mutations in the Algerian population. It remains important for a first screening as it reveals the most common mutations. All this information is of interest for genetic testing and genetic counseling in Algeria and in European countries where immigration from the Maghreb is common.

2015-06-01T00:00:00Z